How To Perform The Delta-Delta Ct Method (In Excel)

This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.

🎓 MASTERING QPCR ONLINE COURSE 🎓
https://courses.toptipbio.com/p/maste...

In this video tutorial, I will show you how to perform the delta-delta Ct method by using Microsoft Excel.

The delta-delta Ct method is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR).

THE ONLINE GUIDE
http://toptipbio.com/delta-delta-ct-pcr/

VIDEO BREAKDOWN
Step 1: Average the Ct values ( 00:53)
Step 2: Calculate delta Ct ( 01:59)
Step 3: Calculate the average delta Ct for controls ( 02:46)
Step 4: Calculate delta-delta Ct ( 03:39)
Step 5: Calculate 2^-(delta-delta Ct) ( 04:54)

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How To Perform The Delta-Delta Ct Method (In Excel) tutorialsystems
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