How To Create Real-Time PCR Primers Using Primer-BLAST

This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.

🎓 MASTERING QPCR ONLINE COURSE 🎓
https://courses.toptipbio.com/p/maste...

In this video tutorial, I will show you how to create real-time PCR primers by using NCBI's Primer-BLAST free online software.

The Primer-BLAST software is great since it uses the Primer3 algorithm to design optimal primers, while simultaneously blasting these sequences on the genome. This ensures the primers will not amplify off-target regions.

THE ONLINE GUIDE
http://toptipbio.com/real-time-pcr-pr...

NCBI WEBSITE
https://www.ncbi.nlm.nih.gov/

VIDEO BREAKDOWN
Step 1: Finding your gene variant ( 00:18)
Step 2: Loading Primer-BLAST ( 01:53)
Step 3: The Primer-BLAST settings ( 02:22)
Step 4: The primer reports ( 08:40)

MORE HELPFUL HINTS & TIPS
http://toptipbio.com/

FOLLOW US
Facebook: https://www.facebook.com/TopTipBio/
Twitter: https://twitter.com/TopTipBio
LinkedIn: https://www.linkedin.com/company/top-...

How To Create Real-Time PCR Primers Using Primer-BLAST bioscience peru
380 Likes	380 Dislikes
24,000 views views	1.41K followers
How-to & Style	Upload TimePublished on 14 Feb 2018

Related keywords

1. research medical center
2. bioscience reports
3. research assistant jobs
4. statistics for data science
5. bioscience argentina
6. bioscience reports impact factor
7. statistics software
8. statistics definition
9. bioscience esteroides
10. research associate
11. statistics formulas
12. research and development
13. bioscience venezuela
14. bioscience srl
15. research paper topics
16. research paper
17. http://bioscience.org
18. bioscience biotechnology and biochemistry
19. bioscience inc
20. research analyst
21. bioscience reports bioxbio
22. statistics for dummies
23. statistics jobs
24. research triangle park
25. statistics terms
26. research paper outline
27. biosciences ltda
28. statistics class
29. bioscience abbreviation
30. bioscience sa
31. research assistant salary
32. statistics synonym
33. bioscience chile
34. research in spanish
35. research design
36. bioscience gmbh
37. bioscience reactivos
38. bioscience journal
39. statistics cheat sheet
40. statistics and probability
41. statistics canada
42. research methods
43. research assistant
44. research paper example
45. statistics calculator
46. researchgate
47. statistics on abortion
48. statistics major
49. bioscience san luis
50. statistics in medicine
51. statistics 101
52. statistics symbols
53. statistics problems
54. research triangle
55. research synonym
56. research jobs
57. research proposal example
58. bioscience research
59. statistics math